IHPE – GALINIER Richard english

richardIHPE UMR 5244
University of Perpignan via Domitia
52 Avenue Paul Alduy
F-66860 Perpignan Cedex FRANCE
Tel +33(0)4-68-66-21-88
Fax +33(0)4-68-66-22-81
email : richard.galinier@univ-perp.fr
POSITION: Research Engineer CNRS (Molecular biologist)
– Responsible for CNRS training
SCIENTIFIC SOCIAL NETWORKS : (click on the logo)
I am leading parts of research projects dealing with immunobiological interactions in host/pathogen systems. Goals are a) the identification and characterization of key molecules involved in pathogen virulence and host resistance, b) to decipher the mechanisms of invertebrate immunity at the molecular and evolution levels, c) to fight against the disease caused by studied pathogens.
Our experimental approaches are mainly comparative analyses of genes and proteins expression between different environmental or experimental conditions, at both individual and population levels. I am also interested by the functional validation of candidate protein identified.
My expertise domain concern transcriptomic and proteomic approaches. At the transcriptomic level: nucleic acid extraction, PCR, cloning, mutagenesis, real time PCR, library construction (EST, microsatellite, substractive,…), in situ hybridization, RNA-Seq and Sanger sequencing analyses, microsatellite multiplex analyses. At the proteomic level: protein 1D electrophoresis, western blot, immunoprecipitation, heterologous and cell free expression system, protein purification system, interactome approach.
2004 – Ph.D Biology (University of Grenoble Joseph Fourier)
1996 – Master Molecular and Cellular Biology, option Biotechnology (University of Strasbourg Louis Pasteur)
1996 – Engineer in Biotechnology, option Molecular and Cellular Biology (Ecole Supérieure de Biotechnologie de Strasbourg)
1993 – Technician in Applied Biology, option Biological and Biochemical Analysis (Technical Institute of the University of La Rochelle)