IHPE – LUVIANO APARICIO Nelia english

NeliaIHPE UMR 5244
University of Perpignan via Domitia
52 Avenue Paul Alduy
F-66860 Perpignan Cedex FRANCE
Tel +33(0)4-68-66-21-81
Fax +33(0)4-68-66-22-81
email : nelia.luviano@univ-perp.fr
POSITION:Ph.D student (Resume french english)
THESIS DIRECTORS:Christoph GRUNAU & Celine COSSEAU
TEAM:ECOEVI
SCIENTIFIC SOCIAL NETWORKS:
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 THESIS SUBJECT:
The DNA methylation of Biomphalaria glabrata, role and impact on the transmission of bilharzia
KEYWORDS:
Epigetics, DNA methylation, mollusc, bilharzia, CRISPR/cas9
THESIS PROJECT:
Biomphalaria glabrata is the intermediate host of schistosomiasis, a parasitic disease provocated by the trematode Schistosoma. The freshwater mollusk is essential for the development of the parasite, therefore a perfect target to prevent its life cycle. Accordingly, knowledge is needed about the host-parasite relationship between the snail and the trematode. During my thesis we will provocate epimutations in the DNA methylation of the snail, an epigenetic mark that seems to be implicated in its reproduction and in the host-parasite interaction. The epimutations will be done by two different methods. 1) The use of DNA methyltranferases inhibitors, the snails are going to be exposed to proprietary chemical substances that will generate random epimutations and we will analyze the phenotype (survival, fecundity, size, color) and the epigenotype (global DNA methylation) of exposed snails. 2) Target epimutations will be provoked in the genome of B. glabrata, there is a technology that allows us to make these changes, its name is CRISPR-Cas9, which is a bacterial immune defense system that makes possible to delete and insert genes at a precise location on the chromosome, within the genome of any cell. This tool will be used to cause DNA methylation changes in targeted sequences and then we will analyze the transcription rates of the targeted genes. The work will aim to provoke dysfunctions of DNA methylation with the objective of find the function of DNA methylation in the genome of B. glabrata, which remains unknown, the results will collaborate with the knowledge of DNA methylation function in invertebrates.
RESUME:
2017 : Master Biodiversity, Ecology, Evolution(Université de Perpignan Via Domita)
2016 : Master of Marine Science – Institute of Marine Sciences and Limnology (National Autonomous University of Mexico)
2012 : Bachelor’s degree in Biology – Faculty of Sciences (National Autonomous University of Mexico)
2009 : Baccalaureate in Life Sciences – Baccalaureate N ° 1 « Gabino Barreda » (National Autonomous University of Mexico)
PUBLICATIONS IN THE LAB : link
PUBLICATIONS OUT OF THE LAB:
Vassallo, A., Davila, Y., Luviano, N., Deneb-Amozurrutia, S., Guadalupe Vital, X., Andres Conejeros, C., … & Alvarez, F. (2014). Inventory of invertebrates from the rocky intertidal shore at Montepio, Veracruz, Mexico. Revista Mexicana de Biodiversidad85: 349-362.
Lozano-Álvarez, E., Luviano-Aparicio, N., Negrete-Soto, F., Barradas-Ortiz, C., Aguíñiga-García, S., Morillo-Velarde, P. S., … & Briones-Fourzán, P. (2017). Does reef architectural complexity influence resource availability for a large reef-dwelling invertebrate? Journal of Sea Research128: 84-91.
Mouahid G., Rognon A., de Carvalho Augusto R., Driguez P., Geyer K., Karinshak S., Luviano N., Mann V., Quack T., Rawlinson K., Wendt G., Grunau C. and Moné H. (2018) Transplantation of schistosome sporocysts between host snails: A video guide. Wellcome Open Research 3