IHPE- MULERO Stephen english

StephenIHPE UMR 5244
University of Perpignan via Domitia
52 Avenue Paul Alduy
F-66860 Perpignan Cedex FRANCE
Tel +33(0)4-68-66-20-50
Fax +33(0)4-68-66-22-81
email : stephen.mulero@univ-perp.fr
POSITION:Ph.D student (Resume french)
THESIS DIRECTORS: Jérôme BOISSIER & Olivier REY
TEAM:ECOEVI
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THESIS SUBJECT:
MOLRISK, a new tool for snail-born infectious disease risk assessment in Europe
KEYWORDS:
eDNA ; Metabarcoding ; Diagnostic
THESIS PROJECT:
This thesis project aims to develop an eDNA technic for freshwater snail vectors and human parasites or related animals detection. It is structured around two main axes.
Ax 1. Concerning the snail vectors, we wish to develop a new metabarcoding tool that will enable us to characterize the spatio-temporal distribution of all freshwater snail species which are the vector of human or animal parasitic disease. This method will allow (a) determining the biodiversity of snails with a systematic and comprehensive approach; (b) monitoring the spatial and temporal distribution of mollusks vectors; (c) identifying the biotic interactions (positive or negative) between these mollusks vectors and other aquatic mollusks species; And (d) considerably reduce the costs of prospecting while increasing the sensitivity of detection.
Ax 2. Concerning the parasites, we develop a DNA-based protocol to detect Schistosoma sp. In relation to the recent epidemic of schistosomiasis in Corsica. On the other hand, we are developing the same approaches to detect parasites that are responsible of medical or veterinary disease.
For the detection of vectors and parasites, this thesis implies a laboratory development phase of the diagnostic tool in mesocosm, . Then these protocols will be validated in natura by coupling eDNA analysis and the implementation of “traditional” technic of vectors and parasites research.
RESUME:
2017-present- Ph.D student in Biology, UMR 5244 CNRS-UPVD IHPE
2015-2017- Master of Science in Emergence of parasitic and infectious diseases (University of Montpellier, Montpellier, France)
2013-2015- Bachelor of Science in Biology and Ecology (University of Perpignan Via Domitia, Perpignan, France)
2012- High-School Degree in Science of life and Earth (Canet en Roussillon, France).
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