Exploring the paradox of holobionts: how microbicidal interfaces host stable interactions with bacteria

PEPS Blanc INEE (2016)
Project leader: Delphine Destoumieux-Garzόn
The concept of holobiont has extended the notion of organism to all the microbial communities associated with it, forming a community of species that is proposed to be the real unit of evolution. In this context, the project LIKE INHOSPITALITY proposes to decipher a paradox in biology: How have bacterial populations been able to establish lasting interactions with epithelia, which are microbicidal surfaces long considered as chemical barriers to the interface with the microbial environment? We propose to pose this question in vibrios, a bacterial genus that has developed a remarkable ability to colonize tissues rich in antimicrobial peptides in animal species. We hypothesize that the ability to resist antimicrobial peptides produced by epihelia is a major determinant of establishing lasting interactions between ecological populations of vibrios and host immune tissues, which may explain the paradox of colonization. of these tissues by these populations. We will develop a multidisciplinary approach that will test the hypothesis of a correlation between resistance to AMPs, electrostatic charge of bacterial membranes and association of vibrios with oysters on a material of choice corresponding to 10 ecological populations of vibrios subservient or negatively associated oysters. This exploratory project should make it possible to determine whether resistance to antimicrobial peptides is a decisive factor for the colonization of a particularly hostile ecological niche that is nevertheless permanently colonized by certain vibrio populations.
Partner 1 – Interactions Hôtes-Pathogènes-Environnements (UMR 5244) Ifremer, CNRS, Université de Perpignan Via Domitia, Université de Montpellier : Delphine Destoumieux-Garzon, Guillaume Charrière, Arnaud Lagorce, Philippe Haffner, Laura Chapelet, Daniel Oyanedel-Trigo
Partner 2 – Laboratoire de Biologie Intégrative des Modèles Marins (UMR 8227), Unité Ifremer Pfom. Frédérique Le Roux
Partner 3 – Centre de Biophysique Moléculaire (UPR 4301). CNRS. Agnès Delmas
Partner 4 – Institut des Biomolécules Max Mousseron UMR 5247). CNRS, Université de Montpellier Hervé Cottet