Sodium boric acid electrophoresis buffer

20x SB buffer:
8g NaOH
45g Boric Acid
to 1L with MilliQ water

In this buffer, agarose gels can be run at higher voltage than in TBE/TAE (typically 10 V/cm). DNA fragment separation is therefore faster.


Brody JR, Kern SE. "Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis." Biotechniques. 2004 Feb;36(2):214-6. [PubMed]