Conditions for qPCR primer design with Primer3Plus

© 2013 Celine Cosseau,

• connect to Primer3Plus
• Task: Detection
• Advanced settings:
  • Table of thermodynamic parameters: SantaLucia 1998
  • Salt correction formula: SantaLucia 1998
• Use Product Size Input and ignore Product Size Range and provide range of 100 to 150 bp.