IHPE – GALINIER Richard

richardIHPE UMR 5244
Université de Perpignan via Domitia
52 Avenue Paul Alduy
F-66860 Perpignan Cedex
Tel +33(0)4-68-66-21-88
Fax +33(0)4-68-66-22-81
email : richard.galinier@univ-perp.fr

GRADE : Ingénieur de Recherches CNRS (Biologie moléculaire)
EQUIPE: 2MAP
RESPONSABILITES :
– Responsable du site perpignanais de l’IHPE (2020-2025)
– Responsable des plateformes techniques et fonctionnelles du site de Perpignan
– Membre du Comité de Pilotage de la plateforme Bio-Environnement de l’UPVD
– Représentant élu du collège des ingénieurs, personnels techniques et administratifs permanents ou non au Conseil d’Unité (2015-2020)

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DOMAINE D’EXPERTISE-RECHERCHE :
Je pilote des parties de projet de recherche portant sur les interactions immunobiologiques au sein des systèmes hôte/pathogène. Plusieurs objectifs guident ces projets : a) l’identification et de caractérisation des molécules clés de la virulence du pathogène et de la résistance de l’hôte, b) décortiquer les mécanismes de l’immunité chez les invertébrés et les replacer dans un contexte évolutif, c) lutter contre les infections relatives aux pathogènes étudiés.
Nos approches expérimentales consistent principalement en des analyses comparatives d’expression de gènes et de protéines impliquées dans ces interactions en fonction de conditions environnementales changeantes, et cela tant au niveau des individus que des populations. Je suis également intéressé par la caractérisation fonctionnelle des protéines candidates ainsi identifiées.
Je maitrise un large panel de techniques de biologie moléculaire et de biochimie. Pour la partie transcriptomique: extraction d’ADN, d’ARN et de protéines, PCR, clonage, mutagénèse dirigée, PCR quantitative, construction de banques EST, de banques de micorsatellites, de banques soustractives, hybridation in situ, mais également analyse de séquençage Sanger et de séquençage massif de transcriptome (RNA-seq), analyse de profil microsatellite en multiplex. Pour la partie protéomique : électrophorèse 1D de protéines, Western blot, immunoprécipitation, expression recombinante de protéines et leur purification, approches d’interactome.

CURSUS :
2004 Doctorat en Biologie (Université Joseph Fourier, Grenoble I)
1996 DEA Biologie moléculaire et cellulaire, option Biotechnologie (Université Louis Pasteur, Strasbourg)
1996 Ingénieur en Biotechnologie, option Biologie moléculaire et cellulaire (Ecole Supérieure de Biotechnologie de Strasbourg)
1993 DUT Biologie Appliquée, option Analyses Biologiques et Biochimiques (IUT La Rochelle)

PUBLICATIONS :



34 documents

Article dans une revue

  • Anaïs Portet, Richard Galinier, Damien Lassalle, Alexandre Faille, Benjamin Gourbal, et al.. Hemocyte siRNA uptake is increased by 5′ cholesterol-TEG addition in Biomphalaria glabrata , snail vector of schistosome. PeerJ, PeerJ, 2021, 9, pp.e10895. ⟨10.7717/peerj.10895⟩. ⟨hal-03163875⟩
  • Jean Delmotte, Cristian Chaparro, Richard Galinier, Julien de Lorgeril, Bruno Petton, et al.. Contribution of Viral Genomic Diversity to Oyster Susceptibility in the Pacific Oyster Mortality Syndrome. Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.1579. ⟨10.3389/fmicb.2020.01579⟩. ⟨hal-02915023⟩
  • David Duval, Remi Pichon, Damien Lassalle, Maud Laffitte, Benjamin Gourbal, et al.. A New Assessment of Thioester-Containing Proteins Diversity of the Freshwater Snail Biomphalaria glabrata. Genes, MDPI, 2020, 11, ⟨10.3390/genes11010069⟩. ⟨hal-02431935⟩
  • Damien Lassalle, Guillaume Tetreau, Silvain Pinaud, Richard Galinier, Neil Crickmore, et al.. Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata. Genes, MDPI, 2020, 11, ⟨10.3390/genes11010065⟩. ⟨hal-02431962⟩
  • Camille Huot, Camille Clerissi, Benjamin Gourbal, Richard Galinier, David Duval, et al.. Schistosomiasis vector snails and their microbiota display a phylosymbiosis pattern. Frontiers in Microbiology, Frontiers Media, In press, ⟨10.3389/fmicb.2019.03092⟩. ⟨hal-02440210⟩
  • Annia Alba, David Duval, Jorge Sánchez, Ana Perez, Silvain Pinaud, et al.. The immunobiological interplay between Pseudosuccinea columella resistant/susceptible snails with Fasciola hepatica: Hemocytes in the spotlight. Developmental and Comparative Immunology, Elsevier, 2020, 102, pp.103485. ⟨10.1016/j.dci.2019.103485⟩. ⟨hal-02279344⟩
  • Guillaume Tetreau, Julien Dhinaut, Richard Galinier, Pascaline Audant-Lacour, Sébastien Voisin, et al.. Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor. PLoS Pathogens, Public Library of Science, 2020, 16 (10), pp.e1008935. ⟨10.1371/journal.ppat.1008935⟩. ⟨hal-02994654⟩
  • Anaïs Portet, Silvain Pinaud, Cristian Chaparro, Richard Galinier, Nolwenn Dheilly, et al.. Sympatric versus allopatric evolutionary contexts shape differential immune response in Biomphalaria / Schistosoma interaction. PLoS Pathogens, Public Library of Science, 2019, 15 (3), pp.e1007647. ⟨10.1371/journal.ppat.1007647⟩. ⟨hal-02076267⟩
  • Silvain Pinaud, Anaïs Portet, Jean-François Allienne, Lucid Belmudes, Cécile Saint-Beat, et al.. Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata. Developmental and Comparative Immunology, Elsevier, 2019, 92, pp.238-252. ⟨10.1016/j.dci.2018.12.001⟩. ⟨hal-01952235⟩
  • Anaïs Portet, Richard Galinier, Silvain Pinaud, Julien Portela, Fanny Nowacki, et al.. BgTEP: an antiprotease involved in innate immune sensing in Biomphalaria glabrata. Frontiers in Immunology, Frontiers, 2018, 9, pp.1206. ⟨10.1101/308130⟩. ⟨hal-01838951⟩
  • Julien de Lorgeril, Aude Lucasson, Bruno Petton, Eve Toulza, Caroline Montagnani, et al.. Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters. Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.4215. ⟨10.1038/s41467-018-06659-3⟩. ⟨hal-01898110⟩
  • C. Adema, L. Hillier, C. Jones, E.S. Loker, M. Knight, et al.. Whole genome analysis of a schistosomiasis-transmitting freshwater snail. Nature Communications, Nature Publishing Group, 2017, 8, pp.15451. ⟨10.1038/ncomms15451⟩. ⟨hal-01523953⟩
  • Guillaume Tetreau, Silvain Pinaud, Anaïs Portet, Richard Galinier, Benjamin Gourbal, et al.. Specific Pathogen Recognition by Multiple Innate Immune Sensors in an Invertebrate. Frontiers in Immunology, Frontiers, 2017, 8, pp.1249. ⟨10.3389/fimmu.2017.01249⟩. ⟨hal-01729395⟩
  • Richard Galinier, Emmanuel Roger, Yves Moné, David Duval, Anaïs Portet, et al.. A multistrain approach to studying the mechanisms underlying compatibility in the interaction between Biomphalaria glabrata and Schistosoma mansoni. PLoS Neglected Tropical Diseases, Public Library of Science, 2017, 11 (3), pp.e0005398. ⟨10.1371/journal.pntd.0005398.s005⟩. ⟨hal-01511153⟩
  • Richard Galinier, Guillaume Tetreau, Anaïs Portet, Silvain Pinaud, David Duval, et al.. First characterization of viruses from freshwater snails of the genus Biomphalaria, the intermediate host of the parasite Schistosoma mansoni. Acta Tropica, Elsevier, 2017, 167, pp.196-203. ⟨10.1016/j.actatropica.2016.12.021⟩. ⟨hal-01435053⟩
  • Laurent Héritier, David Duval, Richard Galinier, Anne-Leila Meistertzheim, Olivier Verneau. Oxidative stress induced by glyphosate-based herbicide on freshwater turtles. Environmental Toxicology and Chemistry, Wiley, 2017, 36 (12), pp.3343 - 3350. ⟨10.1002/etc.3916⟩. ⟨hal-01808555⟩
  • Anaïs Portet, Silvain Pinaud, Guillaume Tetreau, Richard Galinier, Céline Cosseau, et al.. Integrated multi-omic analyses in Biomphalaria-Schistosoma dialogue reveal the immunobiological significance of FREP- Sm PoMuc interaction. Developmental and Comparative Immunology, Elsevier, 2017, 75, pp.16 - 27. ⟨10.1016/j.dci.2017.02.025⟩. ⟨hal-01598455⟩
  • Jérôme Boissier, Sébastien Grech-Angelini, B. L. Webster, Jean-François Allienne, Tine Huyse, et al.. Outbreak of urogenital schistosomiasis in Corsica (France): an epidemiological case study. The Lancet Infectious Diseases, New York, NY : Elsevier Science ; The Lancet Pub. Group, 2001-, 2016, 16 (8), pp.971-979. ⟨10.1016/S1473-3099(16)00175-4⟩. ⟨hal-01364002⟩
  • Silvain Pinaud, Julien Portela, David Duval, Fanny Nowacki,, Marie-Aude Olive, et al.. A Shift from Cellular to Humoral Responses Contributes to Innate Immune Memory in the Vector Snail Biomphalaria glabrata. PLoS Pathogens, Public Library of Science, 2016, 12 (1), pp.e1005361. ⟨10.1371/journal.ppat.1005361⟩. ⟨hal-01259482⟩
  • Nolwenn M Dheilly, Fanny Maure, Marc Ravallec, Richard Galinier, Josée Doyon, et al.. Who is the puppet master? Replication of a parasitic wasp-associated virus correlates with host behaviour manipulation. Proceedings of the Royal Society B: Biological Sciences, Royal Society, The, 2015, 282 (1803), pp.20142773. ⟨10.1098/rspb.2014.2773⟩. ⟨hal-01141139⟩
  • David Duval, Richard Galinier, Gabriel Mouahid, Eve Toulza, Jean-François Allienne, et al.. A Novel Bacterial Pathogen of Biomphalaria glabrata: A Potential Weapon for Schistosomiasis Control?. PLoS Neglected Tropical Diseases, Public Library of Science, 2015, 9 (2), pp.e0003489. ⟨10.1371/journal.pntd.0003489.s002⟩. ⟨hal-01162649⟩
  • Nolwenn M Dheilly, David Duval, Gabriel Mouahid, Rémi Emans, Jean-François Allienne, et al.. A family of variable immunoglobulin and lectin domain containing molecules in the snail Biomphalaria glabrata. Developmental and Comparative Immunology, Elsevier, 2015, 48 (1), pp.234-243. ⟨10.1016/j.dci.2014.10.009⟩. ⟨hal-01082870⟩
  • Rodrigue Mintsa Nguema, Juliette Langand, Richard Galinier, Mohamed A Idris, Mahmoud A Shaban, et al.. Genetic diversity, fixation and differentiation of the freshwater snail Biomphalaria pfeifferi (Gastropoda, Planorbidae) in arid lands.. Genetica, Springer Verlag, 2013, 141, pp.171-184. ⟨10.1007/s10709-013-9715-8⟩. ⟨halsde-00823688⟩
  • Richard Galinier, Julien Portela, Yves Moné, Jean-François Allienne, Hélène Henri, et al.. Biomphalysin, a new β pore-forming toxin involved in Biomphalaria glabrata immune defense against Schistosoma mansoni.. PLoS Pathogens, Public Library of Science, 2013, 9 (3), pp.e1003216. ⟨10.1371/journal.ppat.1003216⟩. ⟨halsde-00823685⟩
  • Julien Portela, David Duval, Anne Rognon, Richard Galinier, Jérôme Boissier, et al.. Evidence for Specific Genotype-Dependent Immune Priming in the Lophotrochozoan Biomphalaria glabrata Snail.. Journal of Innate Immunity, Karger, 2013, 5 (3), pp.261-276. ⟨10.1159/000345909⟩. ⟨halsde-00790554⟩
  • Richard Galinier, Steven van Beurden, Elsa Amilhat, Jeannette Castric, Guy Schoehn, et al.. Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel.. Virus Res, 2012, 166 (1-2), pp.1-12. ⟨10.1016/j.virusres.2012.02.020⟩. ⟨halsde-00700373⟩
  • Gabriel Mouahid, Rodrigue Mintsa Nguema, M.A. Idris, M.A. Shaban, S. Al Yafee, et al.. High phenotypic frequencies of complete albinism in wild populations of Biomphalaria pfeifferi (Gastropoda: Pulmonata). Malacologia, 2010, 53 (1), pp.161-166. ⟨halsde-00608656⟩
  • Mathieu Badets, Guillaume Mitta, Richard Galinier, Olivier Verneau. Expression patterns of Abd-A/Lox4 in a monogenean parasite with alternative developmental paths.. Molecular and Biochemical Parasitology, Elsevier, 2010, 173 (2), pp.154-157. ⟨10.1016/j.molbiopara.2010.05.011⟩. ⟨halsde-00504771⟩
  • Emmanuel Roger, Christoph Grunau, Raymond Pierce, Hirohisa Hirai, Benjamin Gourbal, et al.. Controlled chaos of polymorphic mucins in a metazoan parasite (Schistosoma mansoni) interacting with its invertebrate host (Biomphalaria glabrata).. PLoS Neglected Tropical Diseases, Public Library of Science, 2008, 2 (11), pp.e330. ⟨10.1371/journal.pntd.0000330⟩. ⟨halsde-00344615⟩
  • François Guillou, Guillaume Mitta, Richard Galinier, Christine Coustau. Identification and expression of gene transcripts generated during an anti-parasitic response in Biomphalaria glabrata.. Developmental and Comparative Immunology, Elsevier, 2007, 31 (7), pp.657-71. ⟨10.1016/j.dci.2006.10.001⟩. ⟨hal-00170685⟩
  • Richard Galinier, Evelyne Gout, Hugues Lortat-Jacob, Jonathan Wood, Jadwiga Chroboczek. Adenovirus protein involved in virus internalization recruits ubiquitin-protein ligases.. Biochemistry, American Chemical Society, 2002, 41 (48), pp.14299-305. ⟨hal-01061430⟩

Communication dans un congrès

  • Céline Cosseau, Eve Toulza, Richard Galinier, Benjamin Gourbal, Christoph Grunau. A systems biology approach linking genetic, epigenetic, cytoplasmic and holobiont inheritance to understand rapid adaptation of parasitic flatworms (Schistosoma sp.). 66th Annual Meeting of the American Society of Tropical Medicine and Hygiene, Nov 2017, Baltimore, United States. pp.183. ⟨hal-03132820⟩

Poster

  • David Duval, Richard Galinier, Gabriel Mouahid, Eve Toulza, Anne Rognon, et al.. Paenibacillus, a novel pathogen of Biomphalaria glabrata, an intermediate host for schistosomiasis. ImmunInv 2014 (Colloque Immunité des Invertébrés), May 2014, Dijon, France. ⟨hal-01134490⟩

Pré-publication, Document de travail

  • Anaïs Portet, Eve Toulza, Ana Lokmer, Camille Huot, David Duval, et al.. Infection of the Biomphalaria glabrata vector snail by Schistosoma mansoni parasites drives snail microbiota dysbiosis. 2018. ⟨hal-03134818⟩